• Cellular and molecular events and signaling pathways involved in adaptive response of stressed organism.
• Effects of acute and chronic in vivo treatments with sildenafil (Viagra®, medicine for erectile dysfunction and pulmonary hypertension) on function and signaling of Leydig cells and spermatozoa.
• Regulation of homeostasis and signalization of Leydig cells and spermatozoa during aging.
• Circadian function of Leydig cells of adults and during aging.
• Role of LH, glucocorticoids and melatonin as a synchronizer of peripheral biological clock in Leydig cells.
• Role of Leydig cells’ androgen receptors in molecular adaptation during disturbed homeostasis.
• Role of IGF1R/INSR in regulation of mitochondrial biogenesis and testosterone biosynthesis in Leydig cells.
• Regulatory mechanisms of mitochondrial biogenesis in cells of reproductive axis of male and female.
• Molecular markers of mitochondrial biogenesis in different organs (pineal gland, hypothalamus, brain, adenohypophysis, neurohypophysis, heart, liver, white and brown adipose tissue, kidneys, adrenal glands, testes, prostates, ovaries, musculus gastrocnemius, musculus soleus) from normal and animals with disturbed homeostasis.
• Molecular markers of mitochondrial biogenesis and functionality of spermatozoa during disturbed homeostasis.
• Effects of chronic application of metformin (Glucophage®; medicine for diabetes type 2 and insulin resistance) on function and regulation of Leydig cells, spermatozoa, as well as cells of hypothalamus and adenohypophysis from adult, middle aged and old rats.
| Name | Host institution | |
|---|---|---|
Andrić Silvana |
silvana.andric@dbe.uns.ac.rs | Faculty of Science in Novi Sad |
| Baburski Aleksandar | aleksandar.baburski@dbe.uns.ac.rs | Faculty of Science in Novi Sad |
| Kostić Tatjana | tatjana.kostic@dbe.uns.ac.rs | Faculty of Science in Novi Sad |
| Medar Marija | marija.medar@dbe.uns.ac.rs | Faculty of Science in Novi Sad |
| Radovic Sava | sava.radovic@dbe.uns.ac.rs | Faculty of Science in Novi Sad |
| Sokanovic Srdjan | srdjan.sokanovic@dbe.uns.ac.rs | Faculty of Science in Novi Sad |
| Starovlah Isidora | isidora.starovlah@dbe.uns.ac.rs | Faculty of Science in Novi Sad |
• New molecular mechanisms involved in “fight/adaptation” of Leydig cells during disturbed organism homeostasis.
• New ameliorative effects of sildenafil (Viagra®, medicine for erectile dysfunction and pulmonary hypertension) on testosterone biosynthesis and homeostasis of Leydig cells from old male rats.
• Characterization of peripheral biological clock in Leydig cells from adult and aged rats.
• Description of some of biological clock synchronization pathways in Leydig cells.
• First discovery of profile of molecular markers of mitochondrial biogenesis in cells secreting steroid hormones.
· Gak IA, Radovic SM, Dukic AR, Janjic MM, Stojkov-Mimic NJ, Kostic TS, Andric SA (2015): Stress triggers mitochondrial biogenesis to preserve steroidogenesis in Leydig cells. Biochim Biophys Acta 1853:2217-27. IF=5.30
· Stojkov-Mimic NJ, Bjelic MM, Radovic SM, Mihajlovic AI, Sokanovic SJ, Baburski AZ, Janjic MM, Kostic TS, Andric SA (2015): Intratesticular alpha1-adrenergic receptors mediate stress-disturbed transcription of steroidogenic stimulator NUR77 as well as steroidogenic repressors DAX1 and ARR19 in Leydig cells of adult rats. Mol Cell Endocrinol 412:309-319. IF=4.40
· Stojkov NJ, Baburski AZ, Bjelic MM, Sokanovic SJ, Mihajlovic AI, Drljaca DM, Janjic MM, Kostic TS, Andric SA (2014): In vivo blockade of α1-adrenergic receptors mitigates stress-disturbed cAMP and cGMP signaling in Leydig cells. Mol Hum Reprod 20:77-88. IF=3.96
· Stojkov NJ, Janjic MM, Baburski AZ, Bjelic MM, Mihajlovic AI, Drljaca DM, Sokanovic SJ, Kostic TS, Andric SA (2013): Sustained in vivo blockade of alpha1-adrenergic receptors prevented some of stress-triggered effects on steroidogenic machinery in Leydig cells. Am J Physiol Endocrinol Metab 305: E194-E204. IF=4.83
· Stojkov NJ, Janjic MM, Bjelic MM, Mihajlovic AI, Kostic TS, Andric SA (2012): Repeated immobilization stress disturbed steroidogenic machinery and stimulated the expression of cAMP signaling elements and adrenergic receptors in Leydig cells. Am J Physiol Endocrinol Metab 302: E1239-E1251. IF=4.83
· Baburski AZ, Sokanovic SJ, Bjelic MM, Radovic SM, Andric SA, Kostic TS (2016): Circadian rhythm of the Leydig cells endocrine function is attenuated during aging. Exp Gerontol 73:5-13. IF=3.80
· Baburski AZ, Sokanovic SJ, Janjic MM, Stojkov-Mimic NJ, Bjelic MM, Andric SA, Kostic TS (2015): Melatonin replacement restores the circadian behavior in adult rat Leydig cells after pinealectomy. Mol Cell Endocrinol 413:26-35. IF=4.40
· Sokanovic SJ, Janjic MM, Stojkov NJ, Baburski AZ, Bjelic MM, Andric SA, Kostic TS (2014): Age related changes of cAMP and MAPK signaling in Leydig cells of Wistar rats. Exp Gerontol 58C:19-29. IF=3.80
· Sokanovic SJ, Baburski AZ, Janjic MM, Stojkov NJ, Bjelic MM, Lalosevic D, Andric SA, Stojilkovic SS, Kostic TS (2013): The opposing roles of nitric oxide and cGMP in the age-associated decline in rat testicular steroidogenesis. Endocrinology 154:3914-24. IF=4.87
· Andric SA, Janjic MM, Stojkov NJ, Kostic TS (2010): Sildenafil treatment in vivo stimulates Leydig cell steroidogenesis via the cAMP/cGMP signaling pathway. Am J Physiol Endocrinol Metab 299: E544-E550. IF=4.83